Table 1. Equilibrium dissociation constants (KD) for KDL binding MsbA at various temperatures.
Reported are the mean and standard deviation (n=3).
| Temperature(K) | KD1(μM) | KD2(μM) | KD3(μM) | R2* | Χ2* | |
|---|---|---|---|---|---|---|
| WT | 288 | 0.69±0.06 | 2.64±0.16 | 6.60±0.54 | 0.99 | 0.01 |
| 293 | 0.75±0.04 | 2.86±0.23 | 6.94±0.56 | 0.99 | 0.01 | |
| 298 | 0.68±0.05 | 2.53±0.17 | 6.01±0.40 | 0.99 | 0.01 | |
| 303 | 0.43±0.05 | 1.36±0.10 | 3.30±0.23 | 0.96 | 0.06 | |
| R78A | 288 | 1.91±0.17 | 6.18±0.80 | 0.98 | 0.04 | |
| 293 | 1.87±0.18 | 6.18±0.37 | 0.98 | 0.05 | ||
| 298 | 2.00±0.20 | 5.23±0.47 | 0.98 | 0.04 | ||
| 303 | 7.68±0.47 | 5.72±0.21 | 1 | 0 | ||
| R188A | 288 | 2.21±0.17 | 11.01±1.95 | 0.99 | 0.03 | |
| 293 | 2.28±0.12 | 11.62±2.17 | 0.99 | 0.03 | ||
| 298 | 1.98±0.07 | 8.92±0.73 | 0.99 | 0.03 | ||
| 303 | 1.32±0.05 | 4.37±0.13 | 0.95 | 0.12 | ||
| R238A | 288 | 2.48±0.12 | 4.74±0.45 | 0.99 | 0.02 | |
| 293 | 3.55±0.10 | 6.21±0.40 | 1 | 0.01 | ||
| 298 | 4.70±0.07 | 9.66±0.82 | 1 | 0.01 | ||
| 303 | 4.71±0.20 | 9.94±0.40 | 1 | 0.02 | ||
| K243A | 288 | 1.24±0.07 | 7.28±0.78 | 0.99 | 0.03 | |
| 293 | 1.41±0.09 | 7.38±0.53 | 0.99 | 0.02 | ||
| 298 | 1.28±0.05 | 6.65±0.50 | 0.99 | 0.02 | ||
| 303 | 0.74±0.07 | 3.73±0.32 | 0.97 | 0.06 | ||
| K299A | 288 | 2.32±0.06 | 6.07±0.33 | 17.54±6.77 | 1 | 0.01 |
| 293 | 2.35±0.07 | 5.75±0.07 | 16.03±3.18 | 0.99 | 0.02 | |
| 298 | 2.12±0.09 | 5.07±0.12 | 14.01±1.59 | 0.99 | 0.02 | |
| 303 | 1.38±0.01 | 2.91±0.02 | 5.91±0.55 | 0.97 | 0.05 | |
| R78A K299A | 288 | 2.56±0.17 | 7.77±0.11 | 0.98 | 0.06 | |
| 293 | 3.08±0.13 | 8.92±0.39 | 0.99 | 0.03 | ||
| 298 | 3.17±0.17 | 6.78±0.82 | 0.99 | 0.02 | ||
| 303 | 6.91±0.66 | 11.96±2.83 | 0.99 | 0.03 | ||
| R188A R238A | 288 | 5.55±0.87 | 0.99 | 0.02 | ||
| 293 | 8.51±0.67 | 1 | 0.01 | |||
| 298 | 13.01±0.43 | 1 | 0 | |||
| 303 | 11.14±0.23 | 1 | 0 | |||
| R188A K243A | 288 | 1.06±0.02 | 5.84±0.29 | 0.99 | 0.01 | |
| 293 | 1.07±0.02 | 6.07±0.13 | 0.99 | 0.01 | ||
| 298 | 1.05±0.02 | 5.82±0.06 | 0.99 | 0.02 | ||
| 303 | 0.75±0.01 | 4.35±0.17 | 0.99 | 0.03 | ||
| R188A K299A | 288 | 13.86±0.85 | 13.83±3.23 | 0.99 | 0.04 | |
| 293 | 12.55±0.64 | 14.29±2.18 | 0.99 | 0.03 | ||
| 298 | 9.82±0.70 | 11.50±1.04 | 0.98 | 0.05 | ||
| 303 | 4.86±0.44 | 8.56±0.20 | 0.99 | 0.04 | ||
| R238A K243A | 288 | 2.79±0.20 | 7.93±0.47 | 0.98 | 0.06 | |
| 293 | 3.26±0.09 | 7.39±0.50 | 0.99 | 0.03 | ||
| 298 | 6.53±0.2 | 8.97±0.15 | 1 | 0 | ||
| 303 | 7.63±0.26 | 10.78±2.19 | 1 | 0.01 | ||
| R188A R238A K243A | 288 | 20.53±2.25 | 1 | 0 | ||
| 293 | 21.09±2.84 | 1 | 0 | |||
| 298 | 19.80±1.71 | 1 | 0 | |||
| 303 | 13.12±0.81 | 1 | 0 |
These values represent the replicates with the poorest fits.