Table 2. Thermodynamic signatures of KDL interacting with wild-type and mutant MsbA.
Reported are the mean with standard deviation (n=3), and the subscript denotes the nth KDL binding event.
| T(K) | ΔG1(kJ/mol) | ΔH1(kJ/mol) | -TΔS1(kJ/mol) | ΔCp1(kJ/mol/K) | ΔG2(kJ/mol) | ΔH2(kJ/mol) | -TΔS2(kJ/mol) | ΔCp2(kJ/mol/K) | ΔG3(kJ/mol) | ΔH3(kJ/mol) | -TΔS3(kJ/mol) | ΔCp3(kJ/mol/K) | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | 288 | –34.0±0.2 | –36.0±11.6 | 2.0±11.5 | 8.0±1.5 | –30.8±0.1 | –43.2±7.3 | 12.4±7.3 | 10.1±0.9 | –28.6±0.2 | –36.6±8.1 | 8.0±8.0 | 9.4±0.9 |
| 293 | –34.4±0.1 | 4.3±5.4 | –38.6±5.4 | 7.4±1.5 | –31.1±0.2 | 7.9±2.7 | –39.0±2.7 | 9.1±0.8 | –29.0±0.2 | 10.9±3.3 | –39.8±3.3 | 8.5±0.6 | |
| 298 | –35.2±0.2 | 45.0±5.8 | –80.2±5.9 | 8.9±1.4 | –32.0±0.2 | 59.9±2.3 | –91.8±2.2 | 11.7±1.2 | –29.8±0.2 | 59.1±1.8 | –88.9±1.7 | 10.7±1.5 | |
| 303 | –37.0±0.3 | 86.1±12.1 | –123.0±12.4 | 8.3±1.5 | –34.1±0.2 | 112.3±7.1 | –146.4±7.1 | 10.6±1.0 | –31.8±0.2 | 107.7±7.1 | –139.5±7.1 | 9.8±1.1 | |
| R78A | 288 | –31.6±0.2 | 9.6±4.5 | –41.2±4.7 | –2.6±1.1 | –28.8±0.3 | –13.0±19.1 | –15.8±18.8 | 5.0±1.7 | ||||
| 293 | –32.2±0.3 | –3.5±5.1 | –28.6±5.1 | –2.6±1.1 | –29.2±0.1 | 12.0±11.1 | –41.3±11.1 | 5.0±1.7 | |||||
| 298 | –32.5±0.2 | –16.7±9.6 | –15.9±9.6 | –2.6±1.1 | –30.2±0.2 | 37.1±5.9 | –67.2±6.1 | 5.0±1.7 | |||||
| R188A | 288 | –31.2±0.2 | –23.0±8.2 | –8.3±8.1 | 6.4±1.1 | –27.4±0.4 | –39.1±0.5 | 11.8±0.7 | 11.2±1.0 | ||||
| 293 | –31.7±0.1 | 9.4±3.6 | –41.1±3.5 | 6.1±1.2 | –27.7±0.5 | 17.4±4.6 | –45.1±4.2 | 10.7±1.7 | |||||
| 298 | –32.6±0.1 | 42.1±3.7 | –74.6±3.7 | 6.9±0.9 | –28.8±0.2 | 74.4±8.8 | –103.2±8.6 | 12.1±0.7 | |||||
| 303 | –34.1±0.1 | 74.9±8.3 | –109.0±8.3 | 6.6±1.0 | –31.1±0.1 | 131.6±12.8 | –162.7±12.8 | 11.5±0.7 | |||||
| R238A | 288 | –30.9±0.1 | –66.8±3.4 | 35.9±3.3 | 4.8±0.3 | –29.4±0.2 | –57.9±17.9 | 28.6±17.8 | 2.8±2.4 | ||||
| 293 | –30.6±0.1 | –42.6±4.0 | 12.1±3.9 | 4.1±0.3 | –29.2±0.2 | –43.2±6.5 | 13.9±6.5 | 0.9±2.5 | |||||
| 298 | –30.4±0.1 | –17.9±4.8 | –12.6±4.8 | 5.8±0.3 | –28.6±0.2 | –26.8±7.2 | –1.9±7.0 | 5.5±2.4 | |||||
| 303 | –30.9±0.1 | 7.2±5.8 | –38.1±5.9 | 5.1±0.3 | –29.0±0.1 | –9.5±18.6 | –19.5±18.7 | 3.7±2.4 | |||||
| K243A | 288 | –32.6±0.1 | –48.5±7.3 | 15.9±7.2 | 9.9±0.9 | –28.4±0.3 | –31.6±7.3 | 3.3±7.0 | 8.5±0.9 | ||||
| 293 | –32.8±0.2 | 1.5±3.5 | –34.4±3.5 | 9.1±0.8 | –28.8±0.2 | 11.7±3.1 | –40.5±3.0 | 7.3±1.2 | |||||
| 298 | –33.6±0.1 | 52.2±3.9 | –85.9±4.0 | 11.2±1.2 | –29.6±0.2 | 56.0±2.0 | –85.5±2.1 | 10.3±0.6 | |||||
| 303 | –35.6±0.2 | 103.3±8.0 | –138.9±8.2 | 10.3±1.0 | –31.5±0.2 | 100.8±5.6 | –132.3±5.8 | 9.1±0.8 | |||||
| K299A | 288 | –31.1±0.1 | –22.2±9.5 | –8.9±9.4 | 6.4±1.1 | –28.8±0.1 | –18.8±9.6 | –10.0±9.5 | 7.3±1.0 | –26.4±0.9 | –35.6±71.5 | 9.3±70.7 | 11.4±7.1 |
| 293 | –31.6±0.1 | 9.9±3.8 | –41.5±3.9 | 5.6±1.1 | –29.4±0.1 | 18.0±4.6 | –47.4±4.6 | 6.0±1.0 | –27.0±0.5 | 22.7±37.2 | –49.6±37.5 | 9.2±8.6 | |
| 298 | –32.4±0.1 | 42.7±2.1 | –75.1±1.9 | 7.4±1.2 | –30.2±0.1 | 55.9±1.7 | –86.1±1.6 | 9.0±1.1 | –27.7±0.3 | 82.9±7.8 | –110.6±7.9 | 14.7±5.1 | |
| 303 | –34.0±0.1 | 75.8±7.7 | –109.8±7.7 | 6.7±1.2 | –32.1±0.1 | 94.3±6.3 | –126.4±6.3 | 7.8±1.1 | –30.4±0.2 | 144.1±30.5 | –174.5±30.3 | 12.5±6.4 | |
| R78A K299A | 288 | –30.9±0.2 | –36.8±5.6 | 5.9±5.4 | 4.4±0.8 | –28.2±0.1 | –49.1±5.7 | 20.9±5.7 | 12.0±1.4 | ||||
| 293 | –30.9±0.1 | –15.0±2.8 | –16.0±2.8 | 4.4±0.8 | –28.3±0.1 | 10.7±9.6 | –39.1±9.7 | 12.0±1.4 | |||||
| 298 | –31.4±0.1 | 6.8±3.8 | –38.2±3.9 | 4.4±0.8 | –29.5±0.3 | 70.6±15.6 | –100.1±15.9 | 12.0±1.4 | |||||
| R188A R238A | 288 | –29.0±0.4 | –93.3±15.2 | 64.3±14.8 | 7.8±1.3 | ||||||||
| 293 | –28.5±0.2 | –53.3±9.2 | 24.8±9.1 | 5.9±1.6 | |||||||||
| 298 | –27.9±0.1 | –11.6±4.7 | –16.3±4.6 | 10.6±1.0 | |||||||||
| 303 | –28.8±0.1 | 30.9±5.9 | –59.7±5.9 | 8.7±1.2 | |||||||||
| R188A K243A | 288 | –33±0.1 | –20.3±6.0 | –12.6±6.0 | 4.9±0.6 | –28.9±0.1 | –21.3±3.4 | –7.6±3.3 | 4.8±0.5 | ||||
| 293 | –33.5±0.1 | 4.5±3.2 | –38.0±3.2 | 4.0±0.6 | –29.3±0.1 | 2.7±3.4 | –32.0±3.4 | 4.2±0.4 | |||||
| 298 | –34.1±0.1 | 30.1±1.9 | –64.3±1.9 | 6.2±0.7 | –29.9±0.1 | 27.3±5.0 | –57.1±5.0 | 5.6±0.6 | |||||
| 303 | –35.5±0.1 | 56.1±4.1 | –91.7±4.1 | 5.3±0.6 | –31.1±0.1 | 52.0±7.3 | –83.2±7.4 | 5.0±0.5 | |||||
| R188A K299A | 288 | –26.8±0.1 | –15.4±16.2 | –11.4±16.0 | 8.9±2.2 | –26.9±0.6 | –15.7±36.0 | –11.1±35.4 | 5.2±3.8 | ||||
| 293 | –27.5±0.1 | 29.5±5.2 | –57.0±5.2 | 7.8±1.3 | –27.2±0.4 | 10.1±18.9 | –37.3±18.9 | 5.8±6.0 | |||||
| 298 | –28.6±0.2 | 75.2±7.6 | –103.8±7.4 | 10.4±3.5 | –28.2±0.2 | 35.4±6.4 | –63.6±6.3 | 4.4±1.4 | |||||
| 303 | –30.8±0.2 | 121.4±19.9 | –152.2±20.0 | 9.4±2.6 | –29.4±0.1 | 60.5±14.2 | –89.9±14.2 | 5.0±2.8 | |||||
| R238A K243A | 288 | –30.7±0.2 | –42.4±8.3 | 11.8±8.1 | –1.2±0.5 | –28.1±0.1 | 13.2±9.6 | –41.4±9.6 | –3.8±2.4 | ||||
| 293 | –30.8±0.1 | –46.9±6.0 | 16.1±6.0 | –4.7±0.3 | –28.8±0.2 | –5.4±4.5 | –23.4±4.5 | –4.7±1.6 | |||||
| 298 | –29.6±0.1 | –48.4±4.0 | 18.8±4.1 | 3.8±0.8 | –28.8±0.1 | –23.3±17.2 | –5.5±17.2 | –2.5±3.8 | |||||
| 303 | –29.7±0.1 | –48.3±3.4 | 18.6±3.4 | 0.4±0.6 | –28.9±0.6 | –40.7±31 | 11.9±31.5 | –3.4±2.9 | |||||
| R188A R238A K243A | 288 | –25.9±0.3 | –25.2±5.7 | –0.7±5.4 | 6.2±0.3 | ||||||||
| 293 | –26.3±0.3 | 6.5±3.7 | –32.7±3.4 | 5.3±1.4 | |||||||||
| 298 | –26.9±0.2 | 38.9±0.6 | –65.7±0.7 | 7.6±2.5 | |||||||||
| 303 | –28.3±0.2 | 71.7±3.3 | –100±3.3 | 6.7±0.9 |