Table 3. Double mutant cycle analysis of the first KDL binding to wild-type and mutant MsbA.
The ΔΔ values mutant relative to the wild-type protein. Reported are the mean (n=3).
| Temperature(K) | ΔΔG(kJ/mol) | ΔΔH(kJ/mol) | Δ(-TΔS)(kJ/mol) | ΔΔGint(kJ/mol) | ΔΔHint(kJ/mol) | Δ(-ΔTS)int(kJ/mol) | ||
|---|---|---|---|---|---|---|---|---|
| R78A | 288 | 2.4±0.2 | 45.6±12.5 | –43.2±12.4 | ||||
| 293 | 2.2±0.2 | –7.8±7.5 | 10.0±7.5 | |||||
| 298 | 2.7±0.2 | –61.7±11.3 | 64.4±11.3 | |||||
| R188A | 288 | 2.8±0.2 | 13.0±14.2 | –10.2±14.0 | ||||
| 293 | 2.7±0.1 | 5.2±6.5 | –2.5±6.5 | |||||
| 298 | 2.7±0.2 | –3.0±6.9 | 5.6±7.0 | |||||
| 303 | 2.9±0.4 | –11.2±14.7 | 14.1±14.9 | |||||
| R238A | 288 | 3.1±0.2 | –30.9±12.1 | 33.9±11.9 | ||||
| 293 | 3.8±0.1 | –46.9±6.7 | 50.7±6.7 | |||||
| 298 | 4.8±0.1 | –62.9±7.5 | 67.7±7.6 | |||||
| 303 | 6.1±0.4 | –78.8±13.5 | 84.9±13.7 | |||||
| K243A | 288 | 1.4±0.2 | –12.5±13.7 | 13.9±13.5 | ||||
| 293 | 1.5±0.2 | –2.7±6.5 | 4.3±6.5 | |||||
| 298 | 1.6±0.2 | 7.2±7.0 | –5.6±7.1 | |||||
| 303 | 1.4±0.4 | 17.3±14.5 | –15.9±14.8 | |||||
| K299A | 288 | 2.9±0.2 | 13.8±15.1 | –10.9±14.8 | ||||
| 293 | 2.8±0.1 | 5.7±6.6 | –2.9±6.7 | |||||
| 298 | 2.8±0.2 | –2.4±6.1 | 5.2±6.2 | |||||
| 303 | 3.0±0.4 | –10.3±14.3 | 13.3±14.6 | |||||
| R78A | K299A | 288 | 3.2±0.2 | –0.8±12.9 | 4.0±12.6 | 2.2±0.5 | 60.2±23.4 | –58.0±23.1 |
| 293 | 3.4±0.1 | –19.2±6.1 | 22.7±6.1 | 1.6±0.4 | 17.1±11.6 | –15.6±11.8 | ||
| 298 | 3.8±0.2 | –38.2±6.9 | 42.0±7.1 | 1.7±0.4 | –25.8±14.6 | 27.5±14.7 | ||
| R188A | R238A | 288 | 5.0±0.5 | –57.4±19.1 | 62.4±18.7 | 0.9±0.6 | 39.5±26.7 | –38.7±26.2 |
| 293 | 5.9±0.2 | –57.5±10.7 | 63.5±10.5 | 0.6±0.4 | 15.8±14.2 | –15.2±14.1 | ||
| 298 | 7.3±0.2 | –56.6±7.5 | 64.0±7.5 | 0.1±0.4 | –9.2±12.6 | 9.3±12.7 | ||
| 303 | 8.2±0.4 | –55.1±13.5 | 63.4±13.7 | 0.7±0.5 | –34.9±24.0 | 35.6±24.5 | ||
| R188A | K243A | 288 | 1.0±0.2 | 15.7±13.1 | –14.6±12.9 | 3.2±0.5 | –15.1±23.8 | 18.3±23.4 |
| 293 | 0.9±0.1 | 0.3±6.2 | 0.6±6.2 | 3.4±0.2 | 2.2±11.1 | 1.2±11.1 | ||
| 298 | 1.1±0.2 | –14.9±6.0 | 16.0±6.2 | 3.2±0.4 | 19.1±11.5 | –16.0±11.8 | ||
| 303 | 1.4±0.4 | –29.9±12.7 | 31.4±13.0 | 2.8±0.6 | 36.0±24.2 | –33.2±24.7 | ||
| R188A | K299A | 288 | 7.2±0.2 | 20.6±20.0 | –13.4±19.7 | –1.5±0.5 | 6.2±28.8 | –7.7±28.4 |
| 293 | 6.9±0.1 | 25.2±7.5 | –18.3±7.5 | –1.4±0.2 | –14.4±11.9 | 13.0±11.9 | ||
| 298 | 6.6±0.2 | 30.2±9.6 | –23.6±9.6 | –1.1±0.4 | –35.5±13.2 | 34.3±13.3 | ||
| 303 | 6.1±0.4 | 35.3±23.3 | –29.2±23.5 | –0.3±0.6 | –56.8±31.0 | 56.5±31.5 | ||
| R238A | K243A | 288 | 3.4±0.2 | –6.4±14.3 | 9.8±14.1 | 1.1±0.5 | –36.9±23.3 | 38.0±22.8 |
| 293 | 3.6±0.1 | –51.1±8.1 | 54.7±8.1 | 1.8±0.2 | 1.5±12.4 | 0.3±12.4 | ||
| 298 | 5.6±0.2 | –93.4±7.0 | 99.0±7.2 | 0.8±0.4 | 37.7±12.4 | –37.0±12.7 | ||
| 303 | 7.3±0.4 | –134.4±12.5 | 141.6±12.9 | 0.2±0.6 | 72.8±23.4 | –72.6±24.0 | ||