Table 4. Triple mutant cycle analysis of the first KDL binding to wild-type and mutant MsbA.
Shown as described in Table 3.
| Temperature(K) | ΔΔG(kJ/mol) | ΔΔH(kJ/mol) | Δ(-TΔS) (kJ/mol) | ΔΔGint(kJ/mol) | ΔΔHint(kJ/mol) | Δ(-ΔTS)int(kJ/mol) | |||
|---|---|---|---|---|---|---|---|---|---|
| R188A | R238A | 288 | 2.2±0.2 | –70.4±8.9 | 72.6±8.7 | ||||
| 293 | 3.2±0.1 | –62.7±5.4 | 65.9±5.3 | ||||||
| 298 | 4.7±0.1 | –53.7±6.1 | 58.3±6.1 | ||||||
| 303 | 5.4±0.1 | –43.9±10.2 | 49.3±10.3 | ||||||
| K243A | 288 | –1.8±0.2 | 2.6±11.0 | 19.9±10.8 | |||||
| 293 | –1.8±0.2 | –4.9±5.0 | –6.4±5.0 | ||||||
| 298 | –1.6±0.1 | –11.9±5.4 | 10.4±5.4 | ||||||
| 303 | –1.4±0.2 | –18.7±11.5 | 17.3±11.8 | ||||||
| R238A | K243A | 288 | 5.3±0.4 | –2.2±10.0 | 7.5±9.7 | –4.9±0.5 | –65.6±17.4 | 85.0±16.9 | |
| 293 | 5.4±0.4 | –2.9±5.1 | 8.4±4.9 | –4.0±0.5 | –64.7±8.9 | 51.2±8.8 | |||
| 298 | 5.7±0.2 | –3.2±3.8 | 8.9±3.7 | –2.6±0.2 | –62.4±8.9 | 59.8±8.9 | |||
| 303 | 5.8±0.2 | –3.2±8.9 | 9.0±8.9 | –1.8±0.4 | –59.5±17.8 | 57.7±18.0 | |||
| R238A | R188A | 288 | 1.9±0.2 | –26.5±8.9 | 28.4±8.7 | ||||
| 293 | 2.1±0.1 | –10.6±5.4 | 12.8±5.3 | ||||||
| 298 | 2.5±0.1 | 6.3±6.1 | –3.7±6.1 | ||||||
| 303 | 2.2±0.1 | 23.7±10.2 | –21.5±10.3 | ||||||
| K243A | 288 | 0.3±0.2 | 24.4±8.0 | –24.1±7.8 | |||||
| 293 | –0.2±0.1 | –4.2±5.3 | 4.0±5.3 | ||||||
| 298 | 0.8±0.1 | –30.5±6.2 | 31.3±6.2 | ||||||
| 303 | 1.2±0.2 | –55.5±9.9 | 56.7±10.2 | ||||||
| R188A | K243A | 288 | 5.1±0.2 | 41.7±6.6 | –36.6±6.4 | –2.9±0.4 | –43.8±13.7 | 40.9±13.3 | |
| 293 | 4.3±0.4 | 49.1±5.4 | –44.8±5.1 | –2.4±0.4 | –64.0±9.3 | 61.6±9.1 | |||
| 298 | 3.6±0.2 | 56.7±4.9 | –53.2±4.9 | –0.2±0.2 | –81.0±10.0 | 80.8±10.0 | |||
| 303 | 2.6±0.2 | 64.5±6.7 | –61.9±6.9 | 0.8±0.4 | –96.3±15.7 | 97.1±16.0 | |||
| K243A | R188A | 288 | –0.4±0.2 | 28.1±11.0 | –28.5±10.8 | ||||
| 293 | –0.7±0.2 | 3.0±5.0 | –3.7±5.0 | ||||||
| 298 | –0.5±0.1 | –22.1±5.4 | 21.6±5.4 | ||||||
| 303 | 0.1±0.2 | –47.2±11.5 | 47.3±11.8 | ||||||
| R238A | 288 | 1.9±0.2 | 6.1±8.0 | –4.1±7.8 | |||||
| 293 | 2.0±0.1 | –48.4±5.3 | 50.4±5.3 | ||||||
| 298 | 4.0±0.1 | –100.6±6.2 | 104.6±6.2 | ||||||
| 303 | 5.9±0.2 | –151.6±9.9 | 157.5±10.2 | ||||||
| R188A | R238A | 288 | 6.7±0.2 | 23.3±9.2 | –16.6±8.9 | –5.2±0.4 | 10.9±16.4 | –16.0±16.0 | |
| 293 | 6.6±0.4 | 5.0±5.1 | 1.6±4.9 | –5.2±0.5 | –50.4±8.9 | 45.1±8.8 | |||
| 298 | 6.8±0.2 | –13.3±3.9 | 20.1±4.0 | –3.3±0.2 | –109.4±9.2 | 106.1±9.2 | |||
| 303 | 7.3±0.2 | –31.6±8.7 | 38.9±8.9 | –1.3±0.4 | –167.2±17.5 | 165.9±17.9 | |||