Table 6. Thermodynamic signatures of KDL interacting with wild-type and mutant MsbA trapped with ADP and vanadate.
Reported are the mean with standard deviation (n=3), and the subscript denotes the nth KDL binding event.
| T(K) | ΔG1(kJ/mol) | ΔH1(kJ/mol) | -TΔS1(kJ/mol) | ΔCp1 (kJ/mol/K) | ΔG2(kJ/mol) | ΔH2(kJ/mol) | -TΔS2(kJ/mol) | ΔCp2 (kJ/mol/K) | ΔG3(kJ/mol) | ΔH3(kJ/mol) | -TΔS3(kJ/mol) | ΔCp3 (kJ/mol/K) | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | 293 | –35.3±0.1 | 21.9±1.5 | –57.2±1.3 | –33.3±0.2 | 35.0±0.6 | –68.3±0.6 | ||||||
| 298 | –36.3±0.1 | 21.9±1.5 | –58.2±1.3 | –34.4±0.2 | 35.0±0.6 | –69.4±0.6 | |||||||
| 303 | –37.3±0.1 | 21.9±1.5 | –59.2±1.3 | –35.6±0.2 | 35.0±0.6 | –70.6±0.6 | |||||||
| 310 | –38.6±0.1 | 21.9±1.5 | –60.5±1.3 | –37.2±0.2 | 35.0±0.6 | –72.2±0.6 | |||||||
| R188A | 293 | –32.6±0.1 | –6.6±2.6 | –26.0±2.7 | 3.6±0.1 | –30.7±0.1 | –7.2±2.4 | –23.4±2.5 | 4.1±0.2 | –27.8±0.3 | –3.2±21.3 | –24.7±21.1 | 4.1±2.7 |
| 298 | –33.2±0.2 | 11.3±2.6 | –44.5±2.7 | 3.5±0.1 | –31.2±0.1 | 13.4±3.3 | –44.7±3.4 | 4.2±0.3 | –28.5±0.4 | 17.6±12.4 | –46.1±12.3 | 3.6±4.2 | |
| 303 | –34.1±0.2 | 29.2±2.5 | –63.3±2.6 | 3.6±0.1 | –32.2±0.2 | 34.1±4.3 | –66.3±4.5 | 4.1±0.2 | –29.3±0.3 | 37.8±14.8 | –67.1±15.1 | 4.9±1.4 | |
| 310 | –35.8±0.2 | 54.3±2.6 | –90.2±2.8 | 3.6±0.1 | –34.0±0.3 | 63.0±5.8 | –97.0±6.1 | 4.1±0.2 | –31.3±0.5 | 67.9±26.1 | –99.2±26.6 | 4.3±2.2 | |
| R238A | 293 | –32.1±0.2 | 126.8±5.8 | –158.9±5.9 | –28.9±0.4 | 137.7±10.8 | –166.5±10.8 | ||||||
| 298 | –34.8±0.4 | 126.8±5.8 | –161.6±6.0 | –31.7±0.4 | 137.7±10.8 | –169.4±11.0 | |||||||
| 303 | –37.6±0.4 | 126.8±5.8 | –164.3±6.1 | –34.6±0.5 | 137.7±10.8 | –172.2±11.1 | |||||||
| 310 | –41.4±0.5 | 126.8±5.8 | –168.1±6.2 | –38.5±0.7 | 137.7±10.8 | –176.2±11.4 | |||||||
| K243A | 293 | –31.6±0.1 | 96.2±5.1 | –127.9±5.1 | –29.1±0.2 | 111.7±6.9 | –140.8±6.6 | ||||||
| 298 | –33.8±0.1 | 96.2±5.1 | –130.0±5.1 | –31.5±0.1 | 111.7±6.9 | –143.2±6.7 | |||||||
| 303 | –36.0±0.1 | 96.2±5.1 | –132.2±5.3 | –33.9±0.1 | 111.7±6.9 | –145.6±6.9 | |||||||
| 310 | –39.1±0.2 | 96.2±5.1 | –135.3±5.4 | –37.3±0.2 | 111.7±6.9 | –149±7.1 | |||||||
| R188A R238A | 293 | –33.2±0.2 | –10.2±3.0 | –23.0±3.1 | 7.5±1.0 | –29.8±0.1 | –8.2±30.4 | –21.7±30.3 | 8.1±3.5 | ||||
| 298 | –33.9±0.2 | 27.2±2.5 | –61.0±2.6 | 7.5±0.3 | –30.5±0.5 | 31.9±13.7 | –62.4±14.2 | 8.6±4.6 | |||||
| 303 | –35.2±0.2 | 64.5±7.3 | –99.7±7.2 | 7.5±2.1 | –32.0±0.2 | 72.5±4.9 | –104.5±4.8 | 7.4±1.9 | |||||
| 310 | –38.1±0.4 | 116.9±16.4 | –155.0±16.8 | 7.5±1.3 | –35.0±0.2 | 127.7±25.9 | –162.7±25.9 | 7.9±3.0 | |||||
| R188A K243A | 293 | –30.5±0.2 | 92.4±8.6 | –122.9±8.4 | –1.8±0.9 | –27.0±0.7 | 135.4±44.8 | –162.4±44.1 | –4.0±4.0 | ||||
| 298 | –32.3±0.1 | 82.1±4.4 | –114.4±4.3 | –0.1±0.9 | –29.5±0.1 | 114.7±25.2 | –144.2±25.2 | –3.4±4.5 | |||||
| 303 | –34.7±0.1 | 73.4±0.1 | –108.0±0.1 | –4.4±0.8 | –32.0±0.3 | 94.5±5.7 | –126.5±5.6 | –4.9±3.8 | |||||
| 310 | –36.7±0.1 | 55.6±5.7 | –92.3±5.7 | –2.6±0.8 | –34.5±0.1 | 64.4±22.9 | –98.9±22.9 | –4.3±3.9 | |||||
| R238A K243A | 293 | –33.0±0.1 | 8.9±14.2 | –41.9±14.2 | 12.7±2.7 | –30.1±0.1 | 6.5±2.2 | –36.6±2.1 | 10.0±0.7 | ||||
| 298 | –34.2±0.2 | 71.8±7.2 | –106.0±7.4 | 13.2±2.1 | –31.0±0.1 | 56.1±2.1 | –87.1±2.2 | 10.5±0.9 | |||||
| 303 | –36.7±0.2 | 135.3±16.2 | –171.9±16.3 | 11.9±3.5 | –33.1±0.1 | 106.2±5.4 | –139.3±5.5 | 9.1±1.0 | |||||
| 310 | –41.6±0.8 | 222.4±35.8 | –264.0±36.5 | 12.4±2.9 | –37.0±0.3 | 174.4±10.5 | –211.4±10.8 | 9.7±0.7 |