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. 2024 Jan 19;12:RP91953. doi: 10.7554/eLife.91953

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Genetic reagent (D. melanogaster) Oregon-R Bloomington Drosophila Stock Center BDSC:25211
Genetic reagent (D. melanogaster) w1118 Bloomington Drosophila Stock Center BDSC:3605
Genetic reagent (D. melanogaster) w1118; tubP-TIR1-T2A-Gal80.AID Bloomington Drosophila Stock Center BDSC:92470
Genetic reagent (D. melanogaster) y1 w1118; VK00040/TM6B Bloomington Drosophila Stock Center BDSC:9755
Genetic reagent (D. melanogaster) 3.1Lsp2-Gal4ts Armstrong et al., 2014
Genetic reagent (D. melanogaster) 3.1Lsp2-Gal4AGES This paper Fly line maintained in L. Weaver lab
Antibody anti-alpha-spectrin (Mouse monoclonal) Developmental Studies Hybridoma Bank 3A9 (323 or M10-2) IF (3 µg/ml)
Antibody anti-LamC (Mouse monoclonal) Developmental Studies Hybridoma Bank LC28.26 IF (0.8 µg/ml)
Antibody anti-Vasa (Rat monoclonal) Developmental Studies Hybridoma Bank IF (2.15 µg/ml)
Sequence-based reagent Rp49_F1 Weaver and Drummond-Barbosa, 2018 PCR primer CAGTCGGATCGATATGCTAAGC
Sequence-based reagent Rp49_R1 Weaver and Drummond-Barbosa, 2018 PCR primer AATCTCCTTGCGCTTCTTGG
Sequence-based reagent Act5C_F1 This paper PCR primer AGGCCAACCGTGAGAAGATG
Sequence-based reagent Act5C_R1 This paper PCR primer ACATACATGGCGGGTGTGTT
Sequence-based reagent GstD7_F1 This paper PCR primer TATCCACCGTAGAATGGTTTTCG
Sequence-based reagent GstD7_R1 This paper PCR primer CCCATCCAGGAGTTACTTTTGG
Sequence-based reagent GstE3_F1 This paper PCR primer ATGGGAAAACTTACGCTTTACGG
Sequence-based reagent GstE3_R1 This paper PCR primer ACGATCTTGTAGTCGAAGTCCA
Sequence-based reagent Fad2_F1 This paper PCR primer ACCGGAGTGCTTTACGAATCC
Sequence-based reagent Fad2_R1 This paper PCR primer GTGGTCTTCCGATTGCTTAGC
Sequence-based reagent mag_F1 This paper PCR primer GCATCCCGTACTCCCACAAG
Sequence-based reagent mag_R1 This paper PCR primer AGTTACTGAACAATCCGTGCTG
Commercial assay or kit Stanbio Triglyceride Liquicolor kit Thermo Fisher Scientific Cat#SB-2100-430
Commercial assay or kit Invitrogen RNAqueous-4PCR DNA-free RNA isolation for RT-PCR kit Thermo Fisher Scientific Cat#AM1914
Commercial assay or kit NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770L
Commercial assay or kit ApopTag Indirect In Situ Apoptosis Detection Kit Thermo Fisher Scientific Cat#S7110
Chemical compound, drug 1-Naphthaleneacetic acid Santa Cruz Biotechnology Cat#15165-79-4 Dissolved in 1 mM NaOH
Software, algorithm Benchmark Dose Software (BMDS) U.S. Environmental Protection Agency https://www.epa.gov/bmds
Software, algorithm FLIC source code Ro et al., 2014; Ro et al., 2020 https://github.com/PletcherLab/FLIC_R_Code
Software, algorithm MetaboAnalyst 5.0 Pang et al., 2021 https://www.metaboanalyst.ca/
Other Superscript II Reverse Transcriptase Thermo Fisher Scientific Cat# 18064014 Reverse transcriptase used for cDNA synthesis
Other PowerUp SYBR Green Master Mix Thermo Fisher Scientific Cat# A25742 Master mix used for qPCR reactions
Other VECTASHIELD (Antifade Mounting Medium with DAPI) Thermo Fisher Scientific Cat# NC9524612 Anti-fade mounting media with DAPI included