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. 2024 Jan 26;12:RP89762. doi: 10.7554/eLife.89762

Figure 2. Col11a1 and Mmp14 expression in spine.

(A) A heatmap of transcript per million (TPM) values of COL11A1, MMP14, and other published genes associated with adolescent idiopathic scoliosis (AIS). The average TPM value of matrisome genes is represented as MATRISOME. (B) Detection of collagen a1(XI) in P0.5 mouse spine. Immunohistochemistry (IHC) shown at top, with immunofluorescence (IF) staining below. ‘-ab’ refers to negative controls lacking primary antibody (shown at left). Results are representative of N≥3 technical replicates in whole spines. (C) Detection of collagen a1(XI) in P28 mouse spine. Negative antibody IHC control shown at left; antibody-positive IHC shown at right. Enlarged, rotated view of white boxed area shows a biphasic staining pattern. CEP – cartilage endplate; GP – growth plate. Results are representative of N≥3 technical replicates in whole spines.

Figure 2.

Figure 2—figure supplement 1. Immunofluorescence (IF) staining using collagen a1(XI) antibody in P28 ribs (top).

Figure 2—figure supplement 1.

Boxed area shows biphasic staining pattern expanded at right. R – presumed resting zone; P – columnar chondrocytes of presumed proliferating zone; H – presumed hypertrophic zone and chondro-osseous junction. Each image is representative of results in at least three animals.