Table 3. Primers used in this study.

Primer name	Primer sequence 5′ – 3′
Primers for qRT-PCR
Firefly Fwd	GTGTTGGGCGCGTTATTTATC
Firefly Rev	TAGGCTGCGAAATGTTCATACT
CHS5 Fwd	CTGTGGAGGATGCCAATGAA
CHS5 Rev	AGAGGCAATGTCGGTAGTAAAC
DBF4 Fwd	GCAAGGCAAGAAACTGAAGAAG
DBF4 Rev	GATGTGCACCACTTGCTTTG
HRK1 Fwd	GCCATACAGCTCTGTCCATT
HRK1 Rev	AGAGGAAACGGATGCAGATAAG
NET1 Fwd	TGCTTCAGCTTCTTCCTCTTC
NET1 Rev	GGTTACGGGTGGTTTCCTATT
SAG1 Fwd	CCATCCAGTCCCTCATCTTATAC
SAG1 Rev	TGGCACAGAAGGCGTAAA
SVL3 Fwd	CCTCCTACAACCTCTGTTTCAG
SVL3 Rev	GGTACTAGGCGAAGCCATATTC
18S rRNA Fwd	TCACCAGGTCCAGACACAATAAG
18S rRNA Rev	TCTCGTTCGTTATCGCAATTAAGC
ACT1 Fwd	TGTGTAAAGCCGGTTTTGCC
ACT1 Rev	GATACCTCTCTTGGATTGAGCTTC
Primers used for deletion verification and construction of double deletion strains
KanB	CTGCAGCGAGGAGCCGTAAT
KanC	TGATTTTGATGACGAGCGTAAT
Hyg Fwd	CGGATCCCCGGGTTAATTAA
HygC Rev	GAATTCGAGCTCGTTTAAAC
Hyg-B	TTTCGATCAGAAACTTCTCGACA
Hyg-C	TGCTCCGCATTGGTCTTGACC
eIF2A-A	TTCAGCTTCATAGCGATTTATTTTC
eIF2A-B	CATATGATTAATTGAGCCGGTTTAC
eIF2A-C	AGAGTACATAAGTCAACACCCAAGC
eIF2A-D	GACACTCCATATTCATTTATTGCCT
FCY2-A	TATCATTTCCGCTTATCTGACTTCT
FCY2-B	CTAACCTTAACACCAACTTCCTCAA
FCY2-C	TTGAGGAAGTTGGTGTTAAGGTTAG
FCY2-D	AATCAGCAGATTCCATCAAAAGTAG