Fig. 1.

NK cells take up fatty acids and up-regulate CPT1A during viral infection and cancer. (A–C) Mice were infected with MCMV, and splenic NK cells were assessed by flow cytometry on days 0 and 2 PI. Representative histograms and summary graphs show relative uptake of long-chain fatty acids (BODIPY FL-C16) (A), storage of neutral lipids (BODIPY 493/503) (B), and expression of CPT1A (C). Histograms show naive d0 cells (black line), d2 PI (colored line), and fluorescence minus one (FMO) control (gray filled). (D) Representative histogram and summary graph of CPT1A expression in NK cells from a subcutaneous RMA-S tumor or the peripheral blood of a naive mouse. (E) CPT1A expression in peripheral blood NK cells from healthy donors versus AML patients, as measured by the median fluorescence intensity (MFI) of cells. *P < 0.05, **P < 0.01, and ****P < 0.0001; Student’s t test. Data are representative of 2 to 5 independent experiments, 3 to 8 mice per condition.