Figure 1.

Virus recovery from nasopharyngeal and ocular swab samples. Infectious virus was attempted to recovered from PCR positive nasopharyngeal (A, n=17) and ocular (B, n=6) samples. Virus isolation was considered successful when clear cytopathic effect (CPE) was visible latest after two passages and negative when no CPE was visible after two passages. Presence of SARS-CoV-2 in culture supernatant was confirmed via PCR. Shown is success of virus recovery relative to CT-value of initial nasopharyngeal or ocular swab sample. (C) Successful virus isolates for 8 nasopharyngeal and 1 ocular sample were analyzed for total virus via PCR and infectious virus via TCID₅₀ assay. CT-values ≥ 40 were considered negative (vertical dotted lines). Horizontal dotted line in panel (C) indicated limit of detection for TCID₅₀ assay (31.6 TCID₅₀/ml). Non-parametric correlation was determined according to Spearman. (D) Table summarizing characteristics of isolates shown in Panel (C) and underlying swab samples.