FIG. 2.

RT-PCR product made from rPIV3 vRNA. vRNA was isolated from virions grown in LLC-MK2 cells infected with either wt rPIV3 or the indicated mutant rPIV3. The RNA was subjected to reverse transcription (+) or was mock treated without enzyme (−), followed by PCR with L-specific primers that flank the SphI and NheI sites and yield a 2,951-bp cDNA. M, marker DNA (a mixture of lambda DNA digested with HindIII and φX174 DNA digested with HaeIII). Nucleotide lengths are indicated for several marker bands (in base pairs).