Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 4;151(5):dev202545. doi: 10.1242/dev.202545

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2024. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 6. — scRNA sequencing of day 30 VM neuronal population patterned by F5L6.13 and CHIR99021. (A) UMAP plot showing clustering of the day 30 single cells from one biological replicate. (B) UMAP plot showing the cell identity of treatment conditions (CHIR99021 or F5L6.13) contributing the clusters. (C) Bar plot showing the number of cells from each treatment condition contributing to each cluster. (D) Normalized expression levels of ASCL1 and SNAP25 superimposed onto the UMAPs. Data are colored according to scaled expression in each cell. (E) UMAP plot showing subclustered neuronal population. (F,G) Normalized expression levels of TH (F) and NR4A2 (G) superimposed onto the neuronal subclustered UMAP. Data are colored according to expression levels in each cell. (H) Pie chart displaying the proportion of neuronal subclusters contributed from each treatment condition with the DA⁺ clusters highlighted. The percentage of DA⁺ cluster in each condition is indicated. (I) HiDDEN continuous scoring of the DA neuronal population in CHIR and F5L6.13 conditions. (J) Classification of the DA subpopulation from the continuous score generated from HiDDEN. (K) Gene ontology enrichment analysis of the 394 marker genes of the F5L6_L1 subpopulation. **P<0.01, ***P<0.001, ****P<0.0001 (Fisher's Exact test).