Skip to main content
. 2023 Mar 19;37(4):e22851. doi: 10.1096/fj.202201489RR

FIGURE 3.

FIGURE 3

Life span, motor activity, and oxygen consumption rate in mcu‐1 mutants. (A) Survival curve of WT (ccIs4251; aceIs1) and mcu‐1(ju1154) worms. Data are presented as the mean ± standard error from four plates with 25–30 worms per plate. (B) Mean velocity of WT and mcu‐1(ju1154) worms (n = 16–22 worms). Loss‐of‐function mutation in mcu‐1 ameliorated age‐related reductions in movement velocity. (C) The respiration rate of WT and mcu‐1(ju1154) mutant worms. The respiration rate was measured using a MitoXpress Xtra oxygen consumption assay kit and expressed as the relative fluorescence unit (RFU) per minute per five worms (Materials and Methods). The age‐related reduction was improved in the mutants. Data represent the value from at least 10 repeats, each with five worms. (D) Basal and maximal OCR levels at D7 of WT and mcu‐1(ju1154) at D7 of adulthood. Data represent the value from four repeats, each with at least six worms. Letters on the tops of bars indicate statistical significance by one‐way ANOVA with Dunn's multiple comparison tests (B and C) or Student's t test (D) (**p < .01).