Table 6. Macrolide resistance and quinolone resistance-associated mutations detected by real-time polymerase chain reaction in Mycoplasma genitalium-positive specimens, n=61a.
| Resistance-associated mutations (genes) | Mutations | Real-time polymerase chain reaction results | |
|---|---|---|---|
| n | % | ||
| MRAM (23S rRNA), n=56b |
Wild type | 10 | 18% |
| A2058G | 7 | 12% | |
| A2059G | 39 | 70% | |
| QRAM (parCc), n=55b | Wild type | 39 | 71% |
| S83I (G248T) | 13 | 23% | |
| S83R (A247C) | 2 | 4% | |
| D87Y (G259T) | 1 | 2% | |
Abbreviations: M. genitalium, Mycoplasma genitalium; MRAM, macrolide resistance-associated mutations; QRAM, quinolone resistance-associated mutations; rRNA, ribosomal ribonucleic acid
a The 64 positive samples obtained from the urethral or rectal site were from 61 distinct individuals (three were infected at both sites). For the three participants who were infected at both the urethral and rectal site, the results obtained from the urethral site were used to calculate the prevalence of resistance
b An invalid result (amplification failure) was obtained in five cases for the macrolide assay and six cases for the quinolone assay
c No mutation in the gyrA gene, nor the S83N or the D87N mutations in parC was found