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. 2023 Feb 11;49(3):684–698. doi: 10.1002/biof.1941

FIGURE 5.

FIGURE 5

Analysis of the (Epi‐) Lipidome of Nrf2 deficient murine epidermis. Bar graphs of HPLC‐MS/MS‐based redox lipidomic quantification of the phospholipid oxidation products m/z 814 (PAPC‐OOH*) (A), m/z 790 (PLPC‐OOH*) (B) and m/z 546 (Lyso‐PPC) (C) as an indication of phospholipid peroxidation within the epidermis of young and old wildtype (WT) and Nrf2 deficient (NKO) animals. The effects of Nrf2 deficiency on the abundance of the major classes of stratum corneum (SC) lipids (D) consisting of free fatty acids (FFA, E), cholesterols (Chol, F) and ceramides (Cer, G) was analyzed by high performance thinlayer chromatography. GC–MS analysis: Calculated sum of detected FFA (H) and proportional distribution of detected FFA (I). Asterisks indicate statistically significant differences (*p < 0.05, **p < 0.01, two‐way ANOVA with Tukey's correction for multiple comparisons. Results are depicted as mean with standard deviation). (J) Ratios of relative intensities of genes with lipid metabolism relevant gene ontology terms in transcriptomic analysis data of cultured keratinocytes from WT and NKO murine epidermis. Asterisks indicate statistically significant differences (*p < 0.05, Student's t‐test. Results are depicted as mean with standard error of the mean.)