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. 2022 Nov 11;74(12):1928–1942. doi: 10.1002/art.42285

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© 2022 The Authors. Arthritis & Rheumatology published by Wiley Periodicals LLC on behalf of American College of Rheumatology.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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MicroRNA‐27b‐3p (miR‐27b‐3p) regulates the expression of multiple extracellular matrix (ECM)–related genes. A, Heatmap of genes differentially expressed in reverse transcriptase–quantitative polymerase chain reaction (RT‐qPCR) array among 84 ECM‐related genes found in fibroblast‐like synoviocytes (FLS) isolated from osteoarthritis (OA) synovium that were transfected with miR‐27b‐3p mimic or with control mimic and cultured for 48 hours (n = 4). B, Scatter plots obtained by RT‐qPCR array (screening phase (n = 4) and RT‐qPCR (validation phase, n = 8) comparing relative expression (mean ± SD) of ECM‐related genes (normalized to GAPDH) in OA FLS transfected with miR‐27b‐3p mimic (Mi) or control mimic (Ctrl M) and cultured for 48 hours. Data were log‐transformed before analysis by Student's paired 2‐tailed t‐test. C and D, Left, Immunohistochemical analysis (DAB, brown) of ADAMTS8 (C) and type V collagen (COL5A1) (D) expression in mouse knee joints 10 weeks after destabilization of the medial meniscus (DMM) or sham surgery. Nuclei are counterstained in blue (original magnification × 10). Bottom panels a–c are higher‐magnification views of the boxed areas, with panels a and b showing synovium (original magnification ×20 and ×40) and panel c showing medial tibial plateau cartilage (original magnification ×40). Right, Results plotted as the mean ± SD percentage of synovium or chondrocytes staining positive for ADAMTS8 or COL5A1 in 3 digital images of ×40 fields of view (n = 6 samples/group). * = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001, by Student's unpaired 2‐tailed t‐test with Welch's correction. Color figure can be viewed in the online issue, which is available at http://onlinelibrary.wiley.com/doi/10.1002/art.42285/abstract.