FIGURE 4.

CDDP enhances TPA.TNFα‐mediated medulloblastoma cell death. (A) UW228 medulloblastoma cells, selected by puromycin resistance to stably carry the GRP78.TNFα transgene expression cassette and constitutively express TNFα, were seeded in multiwell plates and allowed to grow for 2 days, and then treated with 1 or 5 μM of CDDP. Cells carrying the GRP78 promoter but no TNFα gene (mock transduction) were also included as control. Cell viability was measured at 16, 24, 48, and 72 h by using the SRB assay. (B) Representative images of stably transduced GRP78.TNFα tumor cells at 72 h posttreatment with 5 μM of CDDP. Scale bar, 100 μm. Data are presented as mean ± SEM (standard error of the mean). ***p ≤ .001. All experiments were repeated twice in triplicate and shown are representative experiments.