FIGURE 5.

CDDP enhances gene expression under the control of CMV and GRP78 promoters. (A) Quantification of TNFα expression by ELISA following CDDP treatment (1 and 5 μM) for 72 h of UW228 medulloblastoma cells transduced with TPA carrying GRP78.TNFα and selected with puromycin to stably express TNFα. Data were normalized to the percentage of viable control parental cells (ctr). Cells transduced with a mock vector lacking the TNFα transgene and treated with CDDP were also included as controls. (B) Daoy and UW228 cells were treated with RGD4C.TPA.GRP78.Lucia or RGD4C.TPA.CMV.Lucia and selected with puromycin. The cells were seeded in multiwell plates and treated with CDDP. Secreted lucia expression was measured 48 h after CDDP treatment and the results are expressed as RLU. Data are presented as mean ± SEM. *p ≤ .05, **p ≤ .01, and ***p ≤ .001. All experiments were repeated twice. Shown are data from representative experiments.