FIGURE 6.

RGD4C.TPA.TNFα systemic targeting of medulloblastoma and biodistribution of TNFα expression. Daoy medulloblastoma cells were subcutaneously implanted into NOD/SCID mice and assigned randomly to either a control group TPA.TNFα (nontargeted, NT) or targeted RGD4C.TPA.TNFα group (RGD4C). (A) Tumor sections from treated mice were stained with primary antibodies for phage (green) and CD31 (red), and also with 0.5 μg/mL DAPI (blue). Scale bar, 50 μm. (B) RNA was extracted from tumors and various healthy tissues of tumor‐bearing mice injected with 5 × 10¹⁰ TU/mouse of targeted (RGD4C) or nontargeted TPA.TNFα. Expression of TNFα was determined by qRT‐PCR relative to GAPDH reference gene. Data are expressed as mean ± SEM. *p ≤ .05.