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. 2023 Jul 28;25(12):2698–2718. doi: 10.1111/1462-2920.16466

FIGURE 7.

FIGURE 7

Pseudomonas stutzeri PS13 uses PCL as a carbon source. (A) Metabolic assay in M9 minimal media containing 0.2 mg/mL 2,3,5‐triphenyltetrazolium chloride to show use of PCL bead as a sole carbon source. Positive controls have 0.4% glucose, negative controls have no carbon source. Representative of six independent repeats for original and enriched communities, three for single isolate, imaged after 7 days growth at 30°C. The PS13 enriched community and single isolate are able to metabolize PCL well, shown by the pink colouring of the PCL surface, whereas the PCL bead is only slightly coloured pink by the original PS13 community. (B) Growth curve of PS13 single isolate P. stutzeri, mean and StEM of four independent repeats in M9 minimal media (no carbon source) with and without PCL. (C) Statistical comparison of growth curve day 28, two‐way ANOVA with Tukey multiple comparisons performed, p‐value <0.0001. (D) Weight loss of PCL from four 28‐day growths with PS13 P. stutzeri, unpaired t‐test, p‐value 0.0018.