Figure 2.

Effect of doxycycline‐induced RIPK3 and Bak induction on (a) HaCaT cell viability (data represent mean normalized to untreated control ±SE, n = 3, *P ˂ 0.05, **P ˂ 0.01), (b) corresponding induction of RIPK3, Bak, and full‐length MLKL expression by doxycycline, (c) effect of RIPK3 and Bak expression on necroptotic and apoptotic markers, and (d) modulation of HMGB1 extracellular release following induction of RIPK3, MLKL, and Bak. HaCaT cells were treated for 6 h with 1 μg/mL doxycycline. Exemplar western blot images selected from n = 3 (b–d). Images have been rearranged to aid clarity. Densitometric analysis was performed on three separate blots and statistical analysis was undertaken using the Mann–Whitney U test (*P ˂ 0.05 and **P ˂ 0.01).