Chemicals, peptides, and recombinant proteins |
|
Tetrodotoxin |
Calbiochem |
554412 |
TNF-α |
Invitrogen |
RRTNFAI |
SB203580 |
Merck Millipore |
152121–47-6 |
SC-514 |
Tocris |
3318 |
JF650X-HaloTag ligand |
HHMI Janelia Research Campus |
N/A |
JF549i-HaloTag ligand |
HHMI Janelia Research Campus |
N/A |
JF635i-HaloTag ligand |
HHMI Janelia Research Campus |
N/A |
Lipofectamine 3000 |
Invitrogen |
L3000001 |
TransIT-LT1 Transfection reagent |
Mirus |
MIR 2300 |
Basic Nucleofector Kit for Primary Mammalian Neurons |
Lonza |
V4XP-3032 |
|
Critical commercial assays |
|
Rat TNF-α ELISA MAXTM Deluxe Set |
BioLegend |
438204 |
|
Experimental models: Cell lines |
|
ND7/23 |
Sigma |
92090903 |
HEK293 |
Sigma |
85120602 |
|
Experimental models: Organisms/strains |
|
Sprague-Dawley rat pups |
Envigo |
N/A |
Nav1.8 KO mice |
Dr. John Wood, UCL |
N/A |
|
Recombinant DNA |
|
hNav1.7 |
This Paper |
N/A |
hNav1.7 Septuple Mutant |
This Paper |
N/A |
hNav1.7 Quadruple Mutant |
This Paper |
N/A |
hNav1.7 Triple Mutant |
This Paper |
N/A |
hNav1.7 T103A |
This Paper |
N/A |
hNav1.7 S110A |
This Paper |
N/A |
hNav1.7 S113A |
This Paper |
N/A |
hNav1.7-Nterminus-Halo |
This Paper |
N/A |
Halo-Nav1.7 |
This Paper |
N/A |
Halo-Nav1.7 S110A |
This Paper |
N/A |
|
Software and algorithms |
|
PatchMaster Next |
HEKA Elektronik |
https://www.heka.com/downloads/downloads_main.html#down_patchmaster_next
|
Python |
Python Software Foundation |
https://www.python.org
|
Prism |
GraphPad Software, Inc |
https://www.graphpad.com/
|
Andor Fusion |
Oxford Instruments |
N/A |
Analyzer |
Sophion |
https://sophion.com/products/high-throughput-screening-on-qube/
|
ViewPoint |
Sophion |
https://sophion.com/products/high-throughput-screening-on-qube/
|
pClamp |
Molecular Devices |
https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite
|
Adobe Illustrator |
Adobe |
N/A |
|
Other |
|
Helios Gene Gun |
Bio-Rad |
1652431 |
Nucleofector IIS |
Lonza |
N/A |
Axopatch 200B |
Molecular Devices |
https://www.moleculardevices.com/products/axon-patch-clamp-system/amplifiers/axon-instruments-patch-clamp-amplifiers
|
MultiClamp 700B |
Molecular Devices |
https://www.moleculardevices.com/products/axon-patch-clamp-system/amplifiers/axon-instruments-patch-clamp-amplifiers
|
HEKA EPC-10 |
HEKA Elektronik |
https://www.heka.com/
|
P97 Pipette Puller |
Sutter Instruments |
N/A |
Qube-384 |
Sophion |
https://sophion.com/products/high-throughput-screening-on-qube/
|
Eclipse TE2000-U inverted microscope |
Nikon |
N/A |
Dragonfly High Speed Confocal Microscope |
Oxford Instruments |
N/A |