FIGURE 3.

Phosphorylated tau (pTau) localized using antibody AT180 (brown pigment), in a young bottlenose dolphin (Tt2). pTau was present within neuropil of the white matter but not the grey matter (a). It was also present in the cytoplasm of nearly all neurons in the cerebrocortical grey matter but absent from the nuclei and surrounding neuropil (b). Furthermore, it was present within the neuropil of the white matter of the supralimbic/paralimbic/limbic lobes of the cortex and the cytoplasm of neurons contained within it (c). Greater magnification of cerebrocortical white matter highlighted the intense cytoplasmic labelling of neurons for pTau while sparing their nuclei (d)