Figure 3.

a) Representative fluorescence confocal microscopy images of live pancreatic islets (from n=3) upon incubation with peptide 7 (green, 10 μM) alone or b) peptide 7 (green, 10 μM) after 1 h pre‐incubation with the unlabeled analogue KP‐10 (50 μM). White arrows point surface and intracellular labeling. c) Representative fluorescence confocal microscopy images of live pancreatic islets (from n=3) after 1 h incubation with peptide 7 (green, 10 μM) and LUXendin645 (red, 100 nM). The membrane‐staining fraction of peptide 7 co‐localizes with the signals of LUXendin645. The agonistic behavior of peptide 7 is featured by the ligand‐mediated internalization of GPR54 receptors. Scale bars: a and b=53 μm, c=34 μm.