Table 1.

β‐D‐Glucuronidase inhibition efficacy by 3–6.

Inhibitor	IC50 [μM] EcGUS[a]	IC50 [μM] AcGH79[a]	IC50 [μM] HPSE[b]	Selectivity factor[c]
1 [d]	0.5±0.1	0.033±3 ⋅ 10−3	>100	N/A
3	>100	21±7	>100	N/A
4	>100	0.156±6 ⋅ 10−3	5±2	20
5	>100	0.167±9 ⋅ 10−3	1.5±0.7	67
6	0.43±0.07	1.43 ⋅ 10−3±0.08 ⋅ 10−3	0.2±0.2	2

[a] 4‐MU fluorogenic substrate assay. [b] In gel competitive ABPP‐based assay. Reported values are mean±S.D. of three technical replicates. [c] Calculated as ratio IC₅₀ EcGUS/IC₅₀ HPSE. [d] Reported values are taken from the literature.[ ²¹ , ²² ]