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. 2022 Dec 30;24(3):146–157. doi: 10.1111/tra.12877

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© 2022 The Authors. Traffic published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Rabies P3 interacts with Treacle dependent on K214/R260. (A, B) HEK‐293T cells transfected to express the indicated GFP proteins without (A) or with (B) co‐expression of HA‐Treacle were subjected to IP using GFP‐Trap, before analysis of by immunoblotting (IB) with the indicated antibodies as previously. ¹¹ (C) HeLa cells transfected to co‐express GFP or GFP‐Treacle with mCh‐P3 or mCh‐P3‐KRm were analysed by live cell CLSM (16–18 h p.t.). Images are representative of ≥7 fields of view; co‐localization of P3 with Treacle is indicated by yellow colouration in merged images.