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. 2022 Dec 30;24(3):146–157. doi: 10.1111/tra.12877

FIGURE 7.

FIGURE 7

RABV P3 does not induce DNA damage. HEK‐293T cells transfected to express GFP or GFP‐RABV‐P3 protein were treated with DMSO or etoposide (which induces DNA double‐stranded breaks) 11 at 21 h p.t. for 3 h prior to (A) fixation and immunostaining for γH2AX phosphorylated at S139 (γH2AX‐p), and imaging by CLSM (images representative of 10 fields of view taken from two independent experiments) or (B) lysis for IB analysis using the indicated antibodies (data representative of three independent experiments).