FIG. 3.

Reduction of ΔΨ_m and increased cell death in circulating PBMC during acute CSF (results for a representative pig). For determination of ΔΨ_m, freshly isolated DiOC₆[3]-labeled PBMC obtained from pigs before infection (n.i.) and 1, 3, 5, and 7 days p.i. (d.p.i.) were analyzed by FCM. The upper row shows the FSC/SSC plots, and the two lower rows show the DiOC₆[3]-PI staining of the cells. A region in the FSC/SSC plots was used to define a gate (G1) for the exclusive analysis of DiOC₆[3]-PI staining of cells with scatter characteristics typical of viable lymphocytes. This is shown in the bottom row of contour plots (gate R1). Regions R1 to R3 defined in the DiOC₆[3]-PI contour plots were used determine the number of DiOC₆[3]^high PI⁻ (R1), DiOC₆[3]^low PI⁻ (R2), and DiOC₆[3]^low PI⁺ (R3) cells.