Figure 2.

Substrates used with the selected MTs indicated. (a) Structures of the THIQs tested. (b) Reaction conversions are based on the consumption of the THIQ analogues 1–14 used, by HPLC analysis against standard calibration curves. Reaction conditions: THIQ 0.5 mM, SAM 3 mM, and selected MTs (clarified lysate, 10 % v/v) which showed conversions after 90 min: RnCOMT in blue, MxSafC in purple, and Cj‐6‐OMT in pink. THIQs (R,S)‐12, (S)‐13, and (S)‐14 were not accepted by any of the MTs tested and are not reported. All reactions were performed in duplicate. See SI for representative HPLC chromatograms and calibration curves.