Figure 4. Gross phenotypic analysis of hspa8^hi138Tg and mab21l2^mw702 (mab21l2^{Arg51_Phe52del}) double mutants.

A-F. Representative images of 48-hpf embryos with various hspa8^hi138Tg and mab21l2^mw702 allelic combinations; specific genotypes (left corner) and assigned grade for coloboma (right) are indicated; retinal margins are indicated with yellow outline. G. Graph of the proportion of embryos per grade to total embryos per allele combination. Grade 0 shown in black, grade 1 in green, grade 2 in blue, grade 3 in purple and grade 4 in red. H. Graph of average lens area per allele combination. Error bars indicate SEM. P values are indicated above each bracket, with significance determined by a P value <0.05. (*** <0.0001; ** <0.0005; * <0.05).