Figure 1. OC cells are highly sensitive to NTX-301 treatment.

(A-C) Effects of NTX-301 on cell viability and estimation of IC₅₀ values in OVCAR5, OVCAR4 and SKOV3 cells (A), OC cells derived from HGSOC tumors (B) and immortalized fallopian tube epithelial cells (FT-190) (C). Cells were treated daily with NTX-301 for 4 days, and cell viability was measured (CCK8 assay) on day 5. (D, E) Colony formation assay (left), and numbers of colonies (mean ± SD, n=3) (right) developed from 1,000 OVCAR5 (D) or OVCAR4 (E) cells treated with different doses of NTX-301 for 7 days. (F, G) Western blot of DNMT1, DNMT3a, DNMT3b, and GAPDH (loading control) in OVCAR5 (F) and SKOV3 (G) cells treated with DMSO, NTX-301(100nM, or 1uM), or decitabine (100nM or 1uM) for 48 hours (n=2 replicates). (H) Western blot of DNMT1 and GAPDH (loading control) in primary OC cells isolated from human HGSOC tumors and treated with DMSO or NTX-301 (100nM or 1uM) for 48 hours (n=2). For all comparisons: *p<0.05, **p<0.01, ***p<0.001.