Figure 4.

Metabolic pathway summarizing the coverage of the C. reinhardtii metabolome (metabolites of interest) from the combined application of NMR and GC–MS. Metabolites that were only identified by NMR are colored blue. Metabolites that were only identified by GC–MS are colored red. Metabolites identified by both methods are colored black, and metabolites that are not identified are colored gray. The embedded Venn diagram identifies the total number of metabolites of interest within these metabolic pathways that were identified either by NMR, by GC–MS, or by both techniques. Reprinted with permission from Bhinderwala, F.; Wase, N.; DiRusso, C.; Powers, R., Combining Mass Spectrometry and NMR Improves Metabolite Detection and Annotation. Analytical Chemistry 2018, 17 (11), 4017–4022. Copyright 2018 American Chemical Society.