| Cas9 crRNA sequence ‘lambda 1’ |
5′-/ AltR 1/rGrUrG rArUrA rArGrU rGrGrA rArUrG rCrCrA rUrGrG rUrUrU rUrArG rArGrC rUrArU rGrCrU / AltR2/-3′ |
| Cas9 crRNA sequence ‘lambda 2’ |
5′-/ AltR 1/rCrUrG rGrUrG rArArC rUrUrC rCrGrA rUrArG rUrGrG rUrUrU rUrArG rArGrC rUrArU rGrCrU / AltR2/-3′ |
| Cas9 crRNA sequence ‘lambda 3’ |
5′-/AltRl /rCrArG rArUrA rUrArG rCrCrU rGrGrU rGrGrU rUrCrG rUrUrU rUrArG rArGrC rUrArU rGrCrU / AltR2/-3′ |
| 3x-biotin-cos1 oligo |
5′-/5Phos/ AGG TCG CCG CCC TT/iBiodT/TT/iBiodT/TT/3BiodT/-3′ |
| 3x-digoxigenin-cos2 oligo |
5′-/5Phos/ GGG CGG CGA CCT TT/iDigN/TT/iDigN/TT/3DigN/-3′ |
| Adapter oligo for lambda DNA dual end biotin labeling |
5′-/5Phos/ GGG CGG CGA CCT TGC A-3′ |
