FIG. 3.

RNase protection assays indicating the kinetics of TK gene transcription and the effect of cycloheximide and ACV on transcription. (A) RNase protection of ORF5 antisense riboprobe (see Fig. 1) when hybridized to infected-cell lysates harvested at various times from 1 to 16 h p.i. (lanes 1h to 16h) or when cultured in the presence of 100 μg of cycloheximide per ml for 8 h (X). (B) Cell lysate RNase protection of the same riboprobe in CCV-infected cell cultures incubated with incremental concentrations of ACV from 0 to 100 μM (lanes 0 through 100). Lane M, molecular size markers; lane P, undigested probe.