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. Author manuscript; available in PMC: 2025 Mar 1.
Published in final edited form as: Microbes Infect. 2023 Dec 21;26(3):105280. doi: 10.1016/j.micinf.2023.105280

Figure 2. Differential cytokine responses in control and SP-R210-deficient macrophages.

Figure 2.

Control, SP-R210L(DN), and SP-R210(KO) cells cultured overnight in FBS replete DMEM were washed and placed in 0.5 mL of 1:1 ratio of serum-free DMEM to PBS in the presence or absence of 20 μg/mL Poly(I:C) or 5 μg/mL Imiquimod. Media were collected for the 2-hour time point and then replaced with 0.5 mL DMEM/10% FBS and media harvested at 2, 6, and 22-hours. The concentration of IFNβ (A,B) and TNFα (C,D) was quantitated by ELISA. N=3. All data are shown as means±SD. (A) **p<003 and ***p<0.0005 for SP-R210L(DN) vs SP-R210L(DN)+pIC at t=4- and 8-hours, respectively. (B) **p<006 and ***p<0.0001 for SP-R210L(DN) vs SP-R210L(DN)+IM at t=4- and 8-hours, respectively. (C) *p<0.015; **p<002, ***p<0.0002 for pIC treated SP-R210L(DN) and SP-R210(KO) cells at t=4-8-hours. (C) *p<0.015; **p<002, ***p<0.0002 for pIC treated SP-R210L(DN) and SP-R210(KO) vs untreated cells at t=4-8-hours. (D) *p<0.05; **p<0.006 for IM treated SP-R210L(DN) and SP-R210(KO) vs untreated cells at t=4-8-hours.