Table 2.
Linear discriminant analysis effect size (LEfSe) all-against-all showing the differentially abundant genes encoding carbohydrate active enzymes (CAZymes)
| Enzyme families | Activity | Enriched group | Lefse | p-value |
|---|---|---|---|---|
| CBM32 | Binding to polygalaturonic acid components of pectin, to galactose and lactose, LacNAc (β-D-galactosyl-1,4-β-D-N-acetylglucosamine) shown for certain bacteria | F_IT | 2.71 | 0.043 |
| CBM0 | Unclassified cmb | F_IT | 2.25 | 0.002 |
| GH84 | N-acetyl β-glucosaminidase (EC 3.2.1.52); hyaluronidase (EC 3.2.1.35); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169) | F_IT | 2.23 | 0.026 |
| GH101 | endo-α-N-acetylgalactosaminidase (EC 3.2.1.97) | F_IT | 2.20 | 0.023 |
| GH85 | endo-β-N-acetylglucosaminidase (EC 3.2.1.96) | F_IT | 2.08 | 0.017 |
| CBM51 | Attached to various enzymes from families GH2, GH27, GH31, GH95, GH98 and GH101 | F_IT | 2.05 | 0.002 |
| AA10 (FORMERLY CBM 33) | Copper-dependent lytic polysaccharide monooxygenases (LPMOs); some proteins have been shown to act on chitin, others on cellulose | F_IT | 1.78 | 0.006 |
| CBM68 | Binding to maltotriose and maltotetraose, binding to galactose for certain bacteria | F_IT | 1.33 | 0.050 |
| GH20 | β-hexosaminidase (EC 3.2.1.52); lacto-N-biosidase (EC 3.2.1.140); β-1,6-N-acetylglucosaminidase (EC 3.2.1.-); β-6-SO3-N-acetylglucosaminidase (EC 3.2.1.-) | F_NL | 3.19 | 0.013 |
| GH89 | α-N-acetylglucosaminidase (EC 3.2.1.50) | F_NL | 2.57 | 0.003 |
| GH110 | α-galactosidase (EC 3.2.1.22); α-1,3-galactosidase (EC 3.2.1.-) | F_NL | 2.40 | 0.018 |
| GH123 | β-N-acetylgalactosaminidase (EC 3.2.1.53); glycosphingolipid β-N-acetylgalactosaminidase (EC 3.2.1.-) | F_NL | 2.37 | 0.001 |
| PL38 | endo-β-1,4-glucuronan lyase (EC 4.2.2.14) | F_NL | 2.15 | 0.004 |
| PL35 | chondroitin lyase / chondroitinase (EC 4.2.2.-) | F_NL | 2.02 | 0.005 |
| PL26 | rhamnogalacturonan exolyase (EC 4.2.2.24) | F_NL | 2.00 | 0.035 |
| CBM67 | L-rhamnose binding activity shown for certain bacteria | F_NL | 2.00 | 0.002 |
| GT101 | β-glucosyltransferase (EC 2.4.1.-) | F_NL | 1.95 | < 0.001 |
| GH50 | β-agarase (EC 3.2.1.81) | F_NL | 1.95 | 0.011 |
| PL15 | alginate lyase (EC 4.2.2.3); oligoalginate lyase / exo-alginate lyase (EC 4.2.2.26); heparin lyase / heparin lyase I (EC 4.2.2.7); heparin-sulfate lyase / heparin lyase III (EC 4.2.2.8) | F_NL | 1.90 | 0.005 |
| PL29 | chondroitin-sulfate ABC endolyase (EC 4.2.2.20) | F_NL | 1.85 | 0.009 |
| GT80 | β-galactoside α-2,6-sialyltransferase (EC 2.4.99.1); β-galactoside α-2,3-sialyltransferase (EC 2.4.99.4) | F_NL | 1.54 | 0.001 |
| CE3 | acetyl xylan esterase (EC 3.1.1.72) | F_NL | 1.44 | 0.009 |
| GH99 | glycoprotein endo-α-1,2-mannosidase (EC 3.2.1.130); mannan endo-1,2-α-mannanase (3.2.1.198) | F_NL | 1.31 | 0.001 |
| GT21 | UDP-Glc: ceramide β-glucosyltransferase (EC 2.4.1.80) | F_NL | 1.13 | 0.049 |
| GH77 | amylomaltase or 4-α-glucanotransferase (EC 2.4.1.25) | M_IT | 3.02 | 0.036 |
| GH25 | lysozyme (EC 3.2.1.17) | M_IT | 2.88 | 0.023 |
| GH120 | β-xylosidase (EC 3.2.1.37) | M_IT | 2.57 | 0.012 |
| CBM22 | A xylan binding function has been demonstrated in several cases and affinity with mixed β-1,3/β-1,4-glucans in one | M_IT | 2.43 | 0.006 |
GH, Glycoside Hydrolases; GT, Glycosyl Transferases; PL, Polysaccharide Lyases; CE, Carbohydrate Esterases; AA, Auxiliary Activities; CBM, Carbohydrate-Binding Modules