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. 2024 Mar 18;43(4):102. doi: 10.1007/s00299-024-03167-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Creation of GhMPK31 transgenic plants with overexpression and RNAi. a Construction of overexpression GhMPK31 vector. b Interference GhMPK31 vector construction. c Genetic transformation process of cotton. d Determination of GhMPK31 expression level in transgenic materials. The expression of GhUB7 was used as internal control. Means ± SE (n = 3). Significant difference analysis was done by Student’s t-tests (**, p < 0.01, ***, p < 0.001). e Phenotypes comparison between T1 transgenic materials and WT plants. Compared with WT plants, OM31 lines showed necrotic lesions in the leaves, a kind of HR-like cell death phenotype. The red line represents 1 cm. f Comparison of plant height between T1 generation OM31 and WT plants. The red line represents 10 cm (colour figure online)