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. 2024 Mar 18;43(4):102. doi: 10.1007/s00299-024-03167-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — GhMPK31 physically interacts with GhRBOHB in cotton. a The subcellular localization of GhMPK31: GFP protein. GFP fluorescence was observed after transiently expressing GhMPK31: GFP protein in tobacco epidermal cells. CBL and HY5 were used as plasma membrane and nucleus markers, respectively. b Y2H assay for GhMPK31- GhRBOHB interaction. SD-2 (-Trp/-Leu), SD-4 (-Trp/-Leu/-His/-Ade). c 3D protein structural model of GhMPK31 and GhRBOHB interaction. d BiFC assay between GhMPK31-cYFP and GhRBOHB-nYFP in tobacco epidermal cells. Bars = 30 μm. e LCI analysis of GhMPK31-nLUC and GhRBOHB-cLUC in tobacco leaves