TABLE 2.
Effects of point mutations in the N-terminal domain on MHV-A59 receptor activity and MAb CC1 binding activitya
| N-terminal domain | Mutation | MHV-A59 receptor activity | MAb CC1 binding |
|---|---|---|---|
| Cea101–70/MHVR71–108 | None | − | − |
| T50S51I52/PNS | − | − | |
| N43G46/KA | − | − | |
| S38G39G41/TTI | +/− | − | |
| S38G39/TT | − | − | |
| G41/I | − | − | |
| Cea101–108 | None | − | − |
| S38G39G41/TTI | − | − |
a
Mutations were designed to change Cea10 amino acids to their corresponding MHVR residues. MHV-A59 receptor activity and MAb CC1 binding activity were determined as described in Materials and Methods.