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. 2024 Mar 5;15:1331156. doi: 10.3389/fpls.2024.1331156

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Copyright © 2024 Zhang, Deneer, Fleck and Hülskamp

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LUMIER binding assays to estimate dissociation constants. (A) Schematic representation of constructs used in LUMIER. (B) Western Blot used to determine the ratio of GL1 and TTG1 to GL1 using peak intensity. (C) Model fit to LUMIER data of GL1 (red) and TTG1 (blue) binding to GL3. The shaded region indicates the 95% confidence interval on the dissociation constant estimate. Error bars indicate 3 technical replicates. Cloud of points stems from three biological replicates which are not averaged into single points due to variation in expression levels.