TABLE 1.
Virus | Cell type used for propagation of virus | Titer determined on:
|
|
---|---|---|---|
Complementing cells | Noncomplementing cells | ||
PrV-Ka | Noncomplementing | 8.8 × 106 | 2.7 × 106 |
Complementing | 6.2 × 106 | 3.6 × 106 | |
PrV-gKβ | Noncomplementing | 8.2 × 104 | 1.2 × 105 |
Complementing | 2.0 × 106 | 2.0 × 106 | |
PrV-gKgB | Noncomplementing | 8.9 × 103 | 6.1 × 103 |
Complementing | 3.0 × 105 | 2.6 × 105 |
Viruses were grown on either gK-complementing or noncomplementing cells, and resulting virus stocks were titrated on complementing and noncomplementing cells. Both gK mutants produced only single infected cells or small foci of infectivity on noncomplementing cells, which were counted as infectious events.