FIG. 1.

Analysis of the ORF 57 promoter. OMK cell monolayers were transfected with 2 μg of pORF57CAT1 by using DOTAP (Boehringer Mannheim), as directed by the manufacturer; cells containing pORF57CAT1 subsequently either remained uninfected or were superinfected with 50 PFU of HVS (strain A11) per cell at 24 h posttransfection. Cells were harvested at 48 h posttransfection, and extracts were assayed for CAT activity. Products from these assays were separated by thin-layer chromotography and detected by autoradiography. Percentages of acetylation were calculated by the scintillation counting of the appropriate regions of the chromatography plate and are shown in graphic format.