FIG. 2.

Mapping the gene responsible for ORF 57 transactivation. OMK cell monolayers were transfected with 2 μg of pORF57CAT1 and a series of plasmids which contained the entire L-DNA region of HVS (strain A11). Cells were harvested at 48 h posttransfection, and extracts were assayed for CAT activity. Products from these assays were separated by thin-layer chromotography and detected by autoradiography. Percentages of acetylation were calculated by the scintillation counting of the appropriate regions of the chromatography plate; error bars indicate the variations among three replicate assays.