FIG. 8.

(a) Summary of gene order in SalHV-1 BamHI P displayed in the same orientation as the genome layout depicted in Fig. 2. Predicted protein coding regions are shaded, and the proposed intron linking ORFs 57 and 58 is shown as a white rectangle. An AATAAA element potentially involved in polyadenylation is shown as a vertical arrow. (b) Alignment of the putative amino acid sequences of SalHV-1 and CCV dUTPases. Conserved residues are shown in the “con” line. Five recognized dUTPase motifs (I to V) are indicated (12). The CCV protein is shown as commencing at the second ATG codon in the relevant reading frame. (c and d) Locations of potential splice sites linking ORFs 57 and 58 in SalHV-1 (c) and CCV (d). The first 600 bp of SalHV-1 BamHI P and the corresponding region in the CCV genome are shown, proceeding from the 3′ end of ORF 57 through the 5′ end of ORF 58. Potential exons and introns are marked. Translated sequences are bracketed by stop codons which define the 3′ and 5′ limits of ORFs 57 and 58, respectively; the two ORFs overlap in CCV. The first ATG codon in ORF 58 is doubly underlined in each sequence. Conserved amino acid residues are singly underlined. Nucleic acid and amino acid residues outside the putative exons are shown in lowercase. AATAAA elements that could signal polyadenylation of transcripts from ORF 57 are underlined. The sequence corresponding to the oligonucleotide used to locate the 3′ end of SalHV-1 ORF 57 is underlined by dots.