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. Author manuscript; available in PMC: 2024 Mar 19.
Published in final edited form as: Methods Mol Biol. 2023;2562:109–122. doi: 10.1007/978-1-0716-2659-7_6

Fig. 1.

Fig. 1

HCR schematic diagram. Schematic of v3.HCR-FISH on tissue sections. Transcripts of interest are hybridized with pools of split initiator probes containing unique initiators, B1–B5 (a). Following hybridization, initiator-specific fluorescently labeled hairpins are applied to the samples and a hybridization chain reaction is initiated (b). Sections are imaged for each gene of interest (c). Probes with hairpins are removed from the sections with a DNase treatment and probe wash (d). Subsequent rounds of hybridization are performed using new probe pools. (Adapted from “Loop-Mediated Isothermal Amplification (LAMP),” by BioRender.com (2021). Retrieved from https://app.biorender.com/biorender-templates)