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. Author manuscript; available in PMC: 2024 Mar 19.
Published in final edited form as: Methods Mol Biol. 2023;2562:109–122. doi: 10.1007/978-1-0716-2659-7_6

Fig. 3.

Fig. 3

Multiplexed FISH. Two sequential rounds of v3.HCR-FISH performed on a regenerating axolotl limb tissue section. The DAPI image is shown for reference (a) along with four genes stained in round 1: Midkine (Mdk) (b), Keratin 5 (Krt5) (c), Axolotl anterior gradient (Aag) (d), Thrombospondin 1 (Thbs1) (e) and an overlay of all four genes (f). Four genes stained in round 2: Hyaluronan And Proteoglycan Link Protein 3 (g), Keratin 17 (h), Methyltransferase-like (i), Laminin beta 1 (j) and an overlay of all 4 genes (k). 50-μm scale bar is included on DAPI image and a dotted line represents the epithelium/mesenchyme boundary