TABLE 2.
Analysis of NC and MA recombinant virions from transfected cell supernatants
| Construct | p24 (ng/ml)a | RT activity/particleb | Infectivityc |
|---|---|---|---|
| pHXB2gpt | 31.41 ± 2.16 | 100 | + |
| pHX/MT-NC | 32.01 ± 2.01 | 97.85 ± 3.05 | − |
| pHX/MT-ZnD | 32.19 ± 0.75 | 91.75 ± 14.25 | − |
| pHX-AD | 29.70 ± 2.61 | 99.25 ± 4.15 | − |
| pHX/MoMA | 27.09 ± 0.45 | 87.40 ± 1.70 | − |
| pHXΔMA | 32.01 ± 0.18 | 87.10 ± 1.20 | − |
Average of three independent experiments ± standard error.
Percentage of wild-type activity; average of two experiments ± standard error. All samples used for RT activity had been adjusted to contain equivalent amounts of p24.
Time course analysis of infections by all viruses was carried out for 32 days after infection at every 4 days. Particle-associated p24 was measured by ELISA, and percentages of HIV-1 antigen-positive cells were measured by immunofluorescence using a mouse monoclonal antibody specific for the p24 Gag protein. Each time point of each culture was evaluated in duplicate in both assays. Dilutions of the supernatant from pHXB2gpt up to 1:10,000 (virus amount equivalent to 3 pg of p24) were still positive in the infectivity assay.