TABLE 3.

Quantitative analysis of HIV-1 genomic RNA in virions derived from NC and MA recombinant viruses^a

Construct	Viral genomic RNA incorporation (% of wild-type level)		Ratio of genomic to spliced RNA determined by RNase protectiond
	RT-PCRb	RNase protectionc
pHXB2gpt	100	100	1:0.10
pHX/MT-NC	49 ± 3	56 ± 14	1:1.10
pHX/MT-ZnD	19 ± 3	17 ± 1	1:1.06
pHX-AD	27 ± 6	20 ± 3	1:1.31
pHX/Mo-MA	11 ± 3	10 ± 4	1:4.31
pHXΔMA	72 ± 2	97 ± 5	1:0.12

^aRelative nucleic acid content of viral particles was measured by RT-PCR and RNase protection. 

^bRT-PCRs using gag-specific primers (HIV1230 and HIVc1686) were carried out to detect viral genomic RNA incorporation (Fig. 4). The average of values ± standard error from three independent experiments is reported for each mutant. 

^cRNase protection analysis was carried out with the HIV-1-specific probe indicated in Fig. 5. The average of values ± standard error from two independent experiments is reported for each mutant. All percentage estimates are rounded to the closest integer. 

^dRatios were evaluated by dividing the number of counts detected in the bands representing spliced messengers by the number of counts detected in the bands representing genomic messengers, after these counts had been normalized to compensate for the difference in size of the two bands (288 bp versus 377 bp).