Figure 8.

SEC profiles indicate that ATF3W_aeg binds ATF3. Shown are size exclusion chromatography profiles for interacting peptides. The peak at approx 19 min for the ATF3-ATF3W_aeg mixture (black trace) represents a dimeric sample while ATF3 (blue trace) and ATF3W_aeg (green trace) generate a peak at approx 20 min, indicating the presence of a monomer. These experiments, undertaken at a total peptide concentration of 20 μM, provide additional evidence for selectivity of the ATF3-ATF3W_aeg interaction. Arrows show controls from previous peptides with elution times for a 32 mer Fos monomeric peptide (20 min) and a 37 mer cJun–FosW heterodimer (18.5 min).¹⁵