FIG. 6.

Displacement of H1 from HPV-11 DNA by E1. Two hundred nanograms of ³²P-labeled HPV-11 DNA restriction fragments were bound to 200 ng of H1; this was followed by incubation with (+) or without (−) the indicated amounts (in micrograms) of E1N or CAT and, finally, digestion with DNase I. H1-bound restriction fragments were protected from digestion by DNase I; displacement of H1 by E1N is indicated by removal of labeled DNAs by DNase I digestion. The image was generated by PhosphorImager analysis (Molecular Dynamics). (B) Linearized map of HPV-11 DNA restriction fragments A to E. Arrows denote viral protein ORFs. The noncoding region between the E6 and L1 ORFs is the URR and contains the HPV-11 origin of DNA replication.